Anti-Atg9A pAb
| Code | Size | Price |
|---|
| MBL-PD042 | 100 ul | £714.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Hamster
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:500 (chemiluminescence detection system) IP - 2.5 uL/300 uL of cell extract from 3 x 106 cells ICC - 1:400
Background:
Autophagosome, which encloses cytoplasmic materials, is formed by coordinated functions of Atg proteins and fuses with lysosome, leading to degradation of enclosed materials. Atg9 is conserved from yeast to mammal and a sole transmembrane protein among core Atg proteins and functions in autophagosome formation (Young et al. 2006). There are two isoforms in mammals, Atg9A and Atg9B. Atg9A, but not Atg9B, is ubiquitously expressed in mouse tissues (Yamada et al. 2005). Atg9A is required for the initial step of autophagosome formation, but exact function is unclear (Itakura et al. 2012).
Formulation:
PBS containing 50% Glycerol (pH 7.2). No preservative is contained.
Gene IDs:
Human: 79065 Mouse: 245860 Rat: 363254
Immunogen Translated:
Mouse Atg9A, 506 aa-839 aa (recombinant)
Shelf Life:
1 year
Source:
Purified Ig from rabbit serum
Target:
Atg9A
References
1) Itakura, E., et al., J. Cell Sci. 125, 1488-1499 (2012) [IC]
2) Young, A. R., et al., J. Cell Sci. 119, 3888-3900 (2006)
3) Yamada, T., et al., J. Bio. Chem. 280, 18283-18290 (2005)