Anti-OsTIR1 pAb
| Code | Size | Price |
|---|
| MBL-PD048 | 100 ul | £345.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Alternative Names:
Oryza sativa Transport inhibitor response 1
Applications:
WB - 1:1,000
Background:
The auxin-inducible degron (AID) system allows the rapid and reversible proteolysis of proteins of interest, which have been invented by Dr. Masato Kanemaki in National Institute of Genetics in Japan. OsTIR1 is a F-box family protein specifically expressed in a rice plant. Since OsTIR1 can function under a culture condition at 37 degree, it is integrated in the AID system to work it in mammalian cells. Briefly, OsTIR1 binds to endogenous Skp1 to form the Skp1-Cullin1-OsTIR1 complex (SCFTIR1). SCFTIR1 binds and ubiquitinates the AID tag in the presence of auxin, and promotes proteasome-dependent degradation of the AID-tagged target protein. Human HCT116 cell lines harboring OsTIR1 (CMV-OsTIR1 RCB4662 or Tet-OsTIR1) are available from the RIKEN Cell Bank.
Conjugate:
Unlabeled
Formulation:
PBS/50% glycerol, pH 7.2
Immunogen Translated:
Recombinant full-length Oryza sativa Transport inhibitor response 1 (OsTIR1) protein
Target:
OsTIR1