Anti-V5-tag pAb-Agarose
| Code | Size | Price |
|---|
| MBL-PM003-8 | Gel: 200 uL | £610.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Immunoprecipitation (IP)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
IP - 20 uL
Background:
Expression vectors containing a protein and a tag peptide are commonly used. V5-Tag fusion protein expression system is preferably used in various laboratories. This specific antibody for V5-Tag fusion protein is a useful tool for monitoring of the fusion protein expression and affinity purification.
Conjugate:
Agarose
Formulation:
400 ug of anti-V5-tag polyclonal antibody covalently coupled to 200 ul of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 ul.*Azide
Immunogen Translated:
Binding synthetic peptide carrier protein (CP-GKPIPNPLLGLDST)
Reactivity:
This antibody recognizes V5-tag
peptide sequence (GKPIPNPLLGLDST) on
Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum
using affinity column. The rabbit was immunized with
carrier protein (CP) conjugated synthetic peptide,
CP-GKPIPNPLLGLDST.
Target:
V5
References
1) Benoki, S., et al., Arch. Biochem. Biophys. 517, 123-130 (2012)
2) Joo, J. Y., et al., Biochem. Biophys. Res. Commun. 406, 627-632 (2011)
3) Miura, S., et al., J. Biol. Chem. 285, 9858-9867 (2010)
4) Maeda, T., et al., Blood 105, 2115-2123 (2005)
5) Gr?ler, M. H., et al., FASEB J. 18, 551-553 (2004)
This antibody is used in these references.