Anti-RFP pAb

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MBL
Product Code: MBL-PM005
Product Group: Primary Antibodies
Supplier: MBL
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MBL-PM005100 ul£253.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
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4°C
Storage:
4°C

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Further Information

Applications:
WB - 1:1000 (chemiluminescence detection system) ICC - 1:500 IHC - It is reported that this antibody can be used in the Immunohistochemistry for frozen section in the reference number 1).
Background:
Expression vectors containing a protein and a tag protein are commonly used. Red Fluorescent Protein (RFP)-Tag fusion protein expression system is preferably used in various laboratories, because its easy monitoring of fusion proteins. This specific antibody for RFP-Tag is useful tool for monitoring of the fusion protein expression.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Immunogen Translated:
RFP
Reactivity:
This antibody reacts with RFP-tagged fusion proteins on Western blotting and Immunocytochemistry. It reacts with DsRed, mRFP1, mCherry, mOrange, mPlum, tdTomato and mStrawberry*. *It is reported in the reference number 1) and 3).
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum. The rabbit was immunized with RFP.
Target:
RFP/DsRed

References

1) Humphreys, B. D., et al., Am. J. Pathol. 176, 85-97 (2010) 2) Skora, A. D., and Spradling, A. C., PNAS 107, 7389-7394 (2010) 3) Ishida, Y., et al., Mol. Biol. Cell 20, 2744-2754 (2009) 4) Bauer, P. O., et al., J. Biol. Chem. 284, 13153-13164 (2009) 5) Fujita, N., et al., Mol. Biol. Cell 19, 4651-4659 (2008) 6) Wong, H. K., et al., Hum. Mol. Genet. 17, 3223-3235 (2008) 7) Fujita, N., et al., Mol. Biol. Cell 19, 2092-2100 (2008) 8) Yomoda, J., et al., Genes Cells 13, 233-244 (2008) 9) von Knethen, A., et al., J. Cell Biol. 176, 681-694 (2007) 10) Matsuura, K., et al., J. Cell Biol. 167, 351-363 (2004) This antibody is used in these references.