Kaede Polyclonal Antibody: Trial Size
Code | Size | Price |
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MBL-PM012MB | 10 ul | £133.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Western Blot (WB)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
WB - 1:1000 (chemiluminesence detection system)
Background:
CoralHue® Kaede protein emits green fluorescence that can be converted to red. The red fluorescence is comparable in intensity to the green and is stable under usual aerobic conditions. The green-to-red conversion is highly sensitive to irradiation with UV or violet light (350-410 nm). Maximal illumination results in a 2,000-fold increase in the ratio of red-to-green signal. The excitation lights used to elicit red and green fluorescence do not induce the photoconversion. This property provides a simple and powerful technique for regional optical marking.
Formulation:
100 ul volume of 10 mM phosphate buffer containing 0.3 M NaCl and 0.09 % NaN3, pH 8.0.
Immunogen Translated:
Full-length protein Kaede
Reactivity:
This antibody reacts with CoralHue® Kaede on Western blotting.
Shelf Life:
1 year
Source:
This antibody was fractioned by salting out and gel filtration on a Sephadex G-20°0 column. The rabbit was immunized with the recombinant CoralHue® Kaede.
Target:
Kaede
References
1) Tanaka, H., et al., Development 134, 3259-3269 (2007)
2) Ando, R., et al., PNAS 99, 12651-12656 (2002)
This antibody is used in reference number 1).