Kaede Polyclonal Antibody: Trial Size

MBL
Product Code: MBL-PM012MB
Product Group: Primary Antibodies
Supplier: MBL
CodeSizePrice
MBL-PM012MB10 ul£133.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Western Blot (WB)
Shipping:
4°C
Storage:
4°C

Documents

Further Information

Applications:
WB - 1:1000 (chemiluminesence detection system)
Background:
CoralHue® Kaede protein emits green fluorescence that can be converted to red. The red fluorescence is comparable in intensity to the green and is stable under usual aerobic conditions. The green-to-red conversion is highly sensitive to irradiation with UV or violet light (350-410 nm). Maximal illumination results in a 2,000-fold increase in the ratio of red-to-green signal. The excitation lights used to elicit red and green fluorescence do not induce the photoconversion. This property provides a simple and powerful technique for regional optical marking.
Formulation:
100 ul volume of 10 mM phosphate buffer containing 0.3 M NaCl and 0.09 % NaN3, pH 8.0.
Immunogen Translated:
Full-length protein Kaede
Reactivity:
This antibody reacts with CoralHue® Kaede on Western blotting.
Shelf Life:
1 year
Source:
This antibody was fractioned by salting out and gel filtration on a Sephadex G-20°0 column. The rabbit was immunized with the recombinant CoralHue® Kaede.
Target:
Kaede

References

1) Tanaka, H., et al., Development 134, 3259-3269 (2007) 2) Ando, R., et al., PNAS 99, 12651-12656 (2002) This antibody is used in reference number 1).