Anti-GST-tag pAb
| Code | Size | Price |
|---|
| MBL-PM013 | 100 ug | £269.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000 IP - 5 uL/sample
Background:
Bacterial Glutathione S-transferase (GST) from Schistosoma japonicum is a widely used protein fusion partner, since it provides exhibits little effect on the biological function of the protein of interest. The GST-tag fusion protein expression system allows for efficient coupling to glutathione (GSH) agarose affinity resins and purification by a single step chromatography. This specific antibody for GST-tag fusion protein is useful for monitoring of the fusion protein expression and affinity purification. It does not recognize mammalian GST.
Formulation:
In PBS/50% glycerol, pH 7.2
Gene IDs:
Human: 373156 Mouse: 56615
Immunogen Translated:
Recombinant GST was purified by column GSH by expressing the pGEX-5X-3 vector
Reactivity:
This antibody reacts with GST on
Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum
using protein A agarose. The rabbit was immunized with
recombinant bacterial glutathione S-transferase, which was
purified from pGEX-5X-3 vector using GSH column.
Target:
GST
References
1) Sato, M., et al., Int. Immunol. 23, 493-502 (2011) [WB]
2) Zhou, J. and Blissard, G. W., J. Virol. 82, 4449-4460 (2008) [WB]
3) Yamaguchi, T., et al., J. Cell Biol. 171, 431-436 (2005)
4) Yamamoto, H., et al., Mol. Cell Biol.19, 3829-3841 (1999)