Anti-Luciferase pAb
| Code | Size | Price |
|---|
| MBL-PM016 | 100 ug | £280.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Firefly
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000 (chemiluminesence detection system) IP - 5 uL ICC - 1:200 IHC - 1:100 (Heat treatment required for paraffin)
Background:
Luciferase is a 61 kDa luminescent enzyme isolated from Photinus pyralis (North American Firefly). The enzyme catalyzes the ATP dependent oxidation of beetle luciferin to produce inorganic phosphate and light. Luciferase is frequently used as a reporter protein to quantify expression levels from promoters in tissue culture and in vivo.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Immunogen Translated:
Length recombinant luciferase (photinus pyralis)
Reactivity:
This antibody reacts with Luciferase
(61 kDa) on Western blotting. It does not cross-react with
the luciferase derived from Renilla mullerei.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum
using protein A agarose. The rabbit was immunized with
recombinant full length luciferase (photinus pyralis).
Target:
Luciferase
References
1) Xu, G., et al., Mol. Pharmacol. 59, 485-492 (2001)