Anti-DDDDK-tag pAb

WB - 1:1,000 (chemiluminescence detection system) IP - 5 uL/sample ICC - 1:1000

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein?s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. The DDDDK epitope tag peptide sequence (XXXDDDDK) was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. The DDDDK peptide has been widely used as a multi-purpose tag, and anti-DDDDK antibodies are optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK fusion proteins.

100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative.

Synthetic peptide binding KLH, DYKDDDDK

This antibody reacts with N-terminal, Internal, and C-terminal DDDDK-tagged protein on Western blotting and Immunoprecipitation.

1 year

This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated synthetic peptide, XXXDDDDK-CP, CP-XXXDDDDK.

DDDDK