Anti-DDDDK-tag pAb-Agarose

IP - 20 uL

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein?s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. The DDDDK epitope tag peptide sequence (DYKDDDDK) was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. The DDDDK peptide has been widely used as a multi-purpose tag, and anti-DDDDK antibody is optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK fusion proteins.

Agarose

320 ug of anti-DDDDK-tagpolyclonal antibody covalently coupled to 200 ul ofagarose gel and provided as a 50% gel slurry suspended inPBS containing preservative (0.09% sodium azide) for atotal volume of 400 ul.

Synthetic peptide binding KLH, DYKDDDDK

This antibody recognizes DDDDK-tag peptide sequence (DYKDDDDK) on Immunoprecipitation.

1 year

This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with KLH conjugated DYKDDDDK peptide

DDDDK