Anti-S-tag pAb
Code | Size | Price |
---|
MBL-PM021 | 100 ug | £242.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Images
Documents
Further Information
Applications:
WB - 1:1,000 (chemiluminescence detection system) IP - 5 uL/sample
Background:
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein?s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-Tag is an epitope tag composed of a 15 residues peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. S-Tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-Tag fusion proteins by enzymatic assays. However, antibody against the S-Tag sequence provides a more convenient and flexible method to easily identify, detect, or purify S-Tag containing fusion proteins.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative.
Immunogen Translated:
Binding synthetic peptide carrier protein (CP-KETAAAKFERQHIDSC)
Reactivity:
This antibody reacts with S-tagged protein on Western blotting and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated synthetic peptide, CP -KETAAAKFERQHIDS.
Target:
S