Anti-His-tag pAb
| Code | Size | Price |
|---|
| MBL-PM032 | 100 ul | £296.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000 IP - 5 uL/sample
Background:
Expression vectors containing a protein and a tag peptide are commonly used. His-Tag fusion protein expression system is preferably used in various laboratories, because its simple protein purification step by heavy metal, such as nickel, affinity chromatography. This specific antibody for His-Tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification.
Formulation:
In PBS/50% glycerol, pH 7.2
Gene IDs:
Human: 57016 Mouse: 15109
Immunogen Translated:
6xHis synthetic peptide carrier protein binding
Reactivity:
This antibody recognizes His-tagged
protein on Western blotting and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum
using affinity column. The rabbit was immunized with
carrier protein (CP) conjugated 6xHis synthetic peptide.
Target:
His
References
1) Nishiyama, A., et al., Nature 502, 249-253 (2013) [WB]
2) Takemoto, M., et al.,Cereb.Cortex 21, 1925-1934 (2011) [IC]
3) Kojima, K., et al., Genes Cells 14, 1155-1165 (2009) [IP]
4) Lee, J. H., et al., J. Virol. 80, 3844-3852 (2006)
5) Muro, S., et al., J. Cell Sci. 116, 1599-1609 (2003)
6) Isoyama, T., et al., J. Biol. Chem. 277, 39634-39641 (2002)
7) Isoyama, T., et al., J. Biol. Chem. 276, 21863-21869 (2001)
8) Toshima, J., et al., Mol. Biol. Cell 12, 1131-1145 (2001)