Anti-His-tag pAb-Agarose
| Code | Size | Price |
|---|
| MBL-PM032-8 | Gel: 200 uL | £307.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Immunoprecipitation (IP)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
IP - 20 uL
Background:
Expression vectors containing a protein and a tag peptide are commonly used. His-Tag fusion protein expression system is preferably used in various laboratories, because its simple protein purification step by heavy metal, such as nickel, affinity chromatography. This specific antibody for His-Tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification.
Conjugate:
Agarose
Formulation:
320 ug of anti-His-tag polyclonal antibody covalently coupled to 200 ul of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 ul.
Immunogen Translated:
6xHis synthetic peptide carrier protein binding
Reactivity:
This antibody recognizes His-tagged protein on Immunoprecipitation
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated?6xHis synthetic peptide.
Target:
His
References
1) Kajikawa, M., et al., J. Virol. 86, 5288-5296 (2012)
2) Shibata, Y., et al., J. Biochem. 150, 679-686 (2011)
3) Takemoto, M., et al., Cereb. Cortex 21, 1925-1934 (2011)
4) Kojima, K., et al., Genes Cells 14, 1155-1165 (2009)
This antibody is used in these references.