Positive control for anti-LC3 antibody
Code | Size | Price |
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MBL-PM036-PN | 100 ul (10 Tests) | £184.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
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Further Information
Applications:
WB - 10 uL/lane (ready-to-use)
Background:
Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
Formulation:
1x105 cells of human LC3B/293T were suspended with 100 ul of Laemmli?s sample buffer.This product contains 5% 2-mercaptoethanol. Handle with care.
Kit Components:
1x100000 cells of human LC3B/293T were suspended with 100 uL of Laemmli?s sample buffer.
This product contains 5% 2-mercaptoethanol. Handle with care.
Shelf Life:
1 year
Target:
LC3