Anti-PIWIL2 (MILI) (Mouse) pAb

WB - 1:1000 (chemiluminescence detection system) IP - 1 uL/sample

The Piwi/Argonaute protein family plays important roles in stem-cell self-renewal, RNA silencing and translational regulation in various organisms. The family members are defined by conserved both PAZ (Piwi Argonaut and Zwille) and Piwi domains participate in RNA binding. Piwi-interacting RNA (piRNA) is a class of small RNA molecules that is expressed uniquely in mammalian testes and forms RNA-protein complexes with Piwi proteins. These small regulatory RNAs have been implicated in spermatogenesis, repression of retrotransposon transposition in germline cells, epigenetic regulation and positive regulation of translation and mRNA stability. MIWI (PIWIL1) and MILI (PIWIL2) are mouse homologues of Piwi. MILI is expressed from primordial germ cells (PGC) in embryo, whereas MIWI is detected later from the mid-pachytene stage to early round spermatids. Their expression overlaps in the mid-pachytene and early round spermatid stage. The expression patterns and knockout phenotypes of mammalian Piwi members indicated MILI is an essential factor for meiotic differentiation during spermatogenesis. It was also reported that MILI (PIWIL2) is widely expressed in various tumors and acts as an oncogene by inhibiting apoptosis and promoting proliferation through the activation of Stat3/Bcl-xL pathway.

100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 55124 Mouse: 57746

Synthetic peptide corresponding to PIWIL2 VRKDREEPRSSLPDPS mouse (107-122 aa)

This antibody reacts with MILI on Western blotting and Immunoprecipitation.

1 year

This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with the synthetic peptide, VRKDREEPRSSLPDPS, corresponding to mouse PIWIL2 (107-122 aa).

PIWIL2