Anti-p62 (SQSTM1) (Human) pAb
| Code | Size | Price |
|---|
| MBL-PM045 | 100 ul | £374.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Hamster
- Human
- Mouse
- Rat
Applications:
- Immuno Electron Microscopy
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000 (chemiluminescence detection system) IP - 2 uL/300 uL of cell extract from 1x107 cells ICC - 1:500 IHC - 1:1000 (Heat treatment required for paraffin)
Background:
p62/SQSTM1 interacts with various molecular groups such as RIP, TRAF6, ERK, aPKCs, and poly-ubiquitin through PB1 domain, Zn finger domain, and UBA domain. This protein direct interacts with LC3, which is localized on autophagosome membrane, and is degradated by autophagic-lysosome pathway. p62 regulates ubiquitin-positive protein aggregates caused by autophagy deficiency.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 8878 Mouse: 18412 Rat: 113894
Immunogen Translated:
Recombinant human p62 (120-440 a.a.)
Reactivity:
This antibody reacts with p62 on Western blotting, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with the recombinant human p62 protein corresponding to amino acids 120-440.
Target:
p62
References
1) Waguri, S., and Komatsu, M., Methods Enzymol. 453, 181-196 (2009)
2) Komatsu, M., et al., Cell 131, 1149-1163 (2007)
3) Moscat, J., et al., Mol. Cell 23, 631-640 (2006)
4) Seibenhener, M. L., et al., Mol. Cell Biol. 24, 8055-8068 (2004)
This antibody is used in the reference number 1).