Anti-Oct3/4 (Mouse) pAb

WB - 1:1000 (chemiluminescence detection system) IP - 2 uL/300 uL of cell extract from 3 x 106 cells ICC - 1:500 IHC - 1:500

The POU family transcription factor Oct-3/4, termed as Oct-3 or Oct-4, encoded by Pou5f1, is expressed in totipotent/pluripotent early embryonic cells. It is also expressed in embryonic stem (ES) cells and embryonal carcinoma (EC) cells, but its expression diminishes when these cells differentiate and lose pluripotency. Oct3/4 contains three functionally characterized domains, the transcriptional activation domain of N- and C-terminal region and the POU DNA-binding domain. The POU domain binds to an octamer sequence, ATTTGCAT. Several target genes of Oct3/4, such as Sox2, contains an octamer element capable of binding Oct3/4. These sites are important for transcriptional activity. Induced pluripotent stem (iPS) cells can be generated from mouse embryonic or adult fibroblasts by induction of four factors, Oct3/4,Sox2, c-Myc, and Klf4. Oct3/4 regulates a expression of Tcl1 (T cell lymphoma break point) and Nanog and contributes to cell proliferation and stabilization of cell pluripotency. Two transcription factors Oct3/4 and Sox2 works together to control a transcriptional regulatory network that regulates the expression of other essential genes.

100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 5460 Mouse: 18999

Recombinant mouse terminal Oct3/4N (1-134 aa)

This antibody reacts with Oct3/4 on Western blotting, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.

1 year

This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with recombinant N-terminal of mouse Oct3/4 corresponding to 1-134 aa.

Oct3/4