Anti-Atg5 pAb
| Code | Size | Price |
|---|
| MBL-PM050 | 100 ul | £307.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:500 (chemiluminescence detection system)
Background:
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg12-Atg5-Atg16L forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 9474 Mouse: 11793
Immunogen Translated:
C-terminal peptide of human Atg5
Reactivity:
This antibody reacts with Atg5-Atg12 complex (55 kDa) on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with the synthetic peptide corresponding to C-terminus of human Atg5.
Target:
Atg5
References
1)
Hanada, T., et al., J. Biol. Chem. 282, 37298-37302 (2007)
2)
Pyo, J. O., et al., J. Biol. Chem. 280, 20722-20729 (2005)
3)
Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)