monomeric Azami-Green 1 (trial size)
Code | Size | Price |
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MBL-PM052MB | 10 ul | £133.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000 IP - 2 uL/Sample ICC - 1:100 IHC - 1:100 (Heat treatment is required)
Background:
CoralHue® Azami-Green (AG) has been cloned from the stony coral, whose Japanese name is?Azami-Sango?. It absorbs light maximally at 492 nm and emits green light at 505 nm. Wild-type CoralHue® AG rapidly matures to form a tetrameric complex. CoralHue® AG has been carefully engineered to form a monomer, CoralHue® monomeric Azami-Green (mAG1) that maintains the brilliance and pH stability of the parent protein. CoralHue® mAG1 can be used to label proteins or subcellular structures. CoralHue® hmAG1 sequence is codon-optimized for higher expression in mammalian cells.
PM052 is available for immunostaining of ?Fucci-S/G2/M Green? (Fucci; Fluorescent Ubiquitination-based Cell Cycle Indicator). Fucci-S/G2/M Green encodes CoralHue® humanized monomeric Azami-Green1 (hmAG1) fused to a part of human Geminin (hGeminin). It is possible to use PM052 for Fucci transgenic strain, B6.Cg-Tg(Fucci)504Bsi mice which express Fucci-S/G2/M Green.
Immunogen Translated:
Recombinant mAG1
Reactivity:
This antibody reacts with CoralHue® monomeric Azami-Green 1 on Western blotting, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with recombinant CoralHue® monomeric Azami-Green 1.
Target:
Azami-Green 1
References
1) Sakaue-Sawano, A., et al., Cell 132, 487-498 (2008)
2) Sakaue-Sawano, A., et al., Chem. Biol. 15, 1243-1248 (2008)