Anti-β-Actin pAb
| Code | Size | Price |
|---|
| MBL-PM053 | 100 ul | £253.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Hamster
- Human
- Mouse
- Rat
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000 (chemiluminescence detection system) IP - 2 uL/200 uL of cell extract from 2 x 106 cells
Background:
Actin is a 42 kDa protein found in eukaryotic cells. Actin is also one of the most highly conserved proteins. Actin participates in many important cellular activities including muscle contraction, cell movement, cell division and the formation of the cytoskeleton. The β-actin exists in most cell types as components of the cytoskeleton.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 60 Mouse: 11461 Rat: 81822
Immunogen Translated:
Synthetic peptide N-terminal β-Actin
Reactivity:
This antibody reacts with human β-actin on western blotting and Immunoprecipitation. The reactivity to mouse, rat, hamster and chicken β-actin was confirmed by Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with KLH conjugated synthetic peptide, corresponding to N-terminus of β-actin.
References
1)
Vandekerckhove, J., and Weber, K.,PNAS 75, 1106-1110 (1978)