Anti-α-Tubulin pAb
| Code | Size | Price |
|---|
| MBL-PM054 | 100 ul | £253.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Hamster
- Human
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000 (chemiluminescence detection system) IP - 2 uL/200 uL of cell extract from 2 x 106 cells ICC - 1:200
Background:
Microtubules are one of the components of the cytoskeleton, which performs essential and diverse functions within eukaryotic cells. Microtubules are composed of a heterodimer of α and β tubulins. Tubulin is a GTP-binding protein, and extension and shortening of the microtubules are regulated by binding/hydrolysis of GTP.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 7846 Mouse: 22142
Immunogen Translated:
Synthetic peptide N-terminal α-Tubulin
Reactivity:
This antibody reacts with human α-tubulin on western blotting and Immunoprecipitation, and with mouse α-tubulin on Immunocytochemistry. The reactivity to rat, hamster and chicken α-tubulin was confirmed by Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with KLH conjugated synthetic peptide, corresponding to N-terminus of α-tubulin.
References
1)
Hall, J. L., and Cowan, N. J., Nucleic Acids Res.13, 207-223 (1985)