Anti-Sox2 (Mouse) pAb
| Code | Size | Price |
|---|
| MBL-PM056 | 100 uL | £345.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1000 (chemiluminescence detection system) IP - 2uL/300 uL of cell extract from 3 x 106 cells ICC - 1:200 IHC - 1:500 (Heat treatment is required)
Background:
Sox2, a member of the SRY-related HMG-box (SOX) family of transcription factors, is expressed in embryonic inner cell mass, epiblast, extraembryonic mesoderm. It is one of the four factors (Oct3/4, Sox2, Klf4, c-Myc) that have been used to form iPS cells (induced pluripotent stem cells) from fibroblasts. Sox2 and Oct3/4 works together to control a transcriptional regulatory network that regulates the expression of other essential genes, and plays important roles in early embryonic development.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative.
Gene IDs:
Human: 6657 Mouse: 20674
Immunogen Translated:
Recombinant mouse Sox2 part
Reactivity:
This antibody reacts with mouse Sox2 (35 kDa) on Western blotting, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. The reactivity to human Sox2 was confirmed by Western blotting and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with partial recombinant mouse Sox2 protein.
Target:
Sox2
References
1)
Niwa, H., Development 134, 635-646 (2007)
2)
Yu, J., et al., Science 318, 1917-1920 (2007)
3)
Masui, S., et al., Nat. Cell Biol. 9, 625-635 (2007)
4)
Avilion, A. A., et al., Genes & Dev. 17, 126-140 (2003)