Anti-Nanog (Mouse) pAb
| Code | Size | Price |
|---|
| MBL-PM058 | 100 ul | £338.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:500 (chemiluminescence detection system) IP - 2uL/300 uL of cell extract from 3 x 106 cells ICC - 1:200
Background:
Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of the blastocyst. Nanog containing a homeobox domain is a transcription factor expressed in ES cells, and it is required for maintenance of pluripotency in mouse epiblast and ES cells. Pluripotency of mouse ES cells can be maintained by leukemia inhibitory factor (LIF), but Nanog is capable of maintaining ES cell self-renewal independently of LIF.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative.
Gene IDs:
Human: 79923 Mouse: 71950
Immunogen Translated:
Recombinant full-length mouse Nanog
Reactivity:
This antibody reacts with mouse Nanog on Western blotting, Immunoprecipitation and Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with the recombinant full length of mouse Nanog.
Target:
Nanog
References
1)
Junying, Yu., et al., Science 318, 1917-1920 (2007)
2)
Mitsui, K., et al., Cell 113, 631-642 (2003)
3)
Chambers, I., et al., Cell 113, 643-655 (2003)