Anti-KDEL pAb
| Code | Size | Price |
|---|
| MBL-PM059 | 100 ul | £336.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Affinity Purified Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunocytochemistry (ICC)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:500 (chemiluminescence detection system) ICC - 1:1000
Background:
The KDEL (lys-asp-glu-leu)
sequence is most common endoplasmic reticulum (ER)
retention signal. The ER resident proteins, which should be
located in the ER, have this retention motif, and this
retention is mediated by KDEL receptor. This antibody can
specifically detect GRP78 (78 kDa protein) and GRP94 (94
kDa protein) including a KDEL sequence by WB.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative.
Gene IDs:
Human: 10945 Mouse: 68137
Immunogen Translated:
Mouse GRP78 C-terminal 644-654 aa
Reactivity:
This antibody reacts with GRP78 and
GRP94 including KDEL sequence for Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum
using affinity column. The rabbit was immunized with the
KLH conjugated synthetic peptide CTGEEDTSEKDEL
corresponding to amino acid residues 644-654 from mouse
GRP78.
Target:
KDEL
References
1) Pelham, H. R., EMBO J. 7, 913-918 (1988)
2) Munro, S., and Pelham, H. R., Cell 48, 899-907 (1987)