Anti-EIF2C2 (AGO2) (Human) mAb

WB - 1-4 ?g/mL for chemiluminescence detection system IP - 1.5 ?g/500 ?L of cell extract from 1.5x 106 cells RIP - 15 ?g/500 ?L of cell extract from 0.85 x 107 cells

Eukaryotic translation initiation factor 2C, subunit 2 (EIF2C2; AGO2), is a member of the argonaute protein family. It is a core component of RNA-induced silencing complex (RISC). The EIF2C2 belonging to the human argonaute protein family possesses endonuclease activity and functions as a slicer in gene silencing pathways. It recognizes and cleaves target mRNA by RNA interference. In small interference RNA (siRNA) pathway, EIF2C2 forms a complex with EIF2C1 and siRNA, and in microRNA (miRNA) pathway, it binds EIF2C3 to miRNA. EIF2C2 expression is controlled by epidermal growth factor receptor and mitogen-activated protein kinase signaling in human breast cancer cell line.

1 mg/mL

Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of RNAs. The RNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit for microRNA distributed from MBLI. Its ability to immunoprecipitate RNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.

200 ug IgG in 200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.

Human: 27161 Mouse: 239528

(180 amino acids. About terminal region of N) EIF2C2 partial-length recombinant

This antibody reacts with human EIF2C2 (~93.6 kDa) on Western blotting and Immunoprecipitation. Other species cross reactivity is confirmed by Western blotting.

1 year

This antibody was purified from hybridoma (clone 1B1-E2H5) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse lymphocyte immunized with human EIF2C2 fusion protein corresponding to N-terminal regions.

EIF2C2/AGO2