Anti-EIF4G2 (Human/Mouse) pAb

WB - 1:1,000 for chemiluminescence detection system IP - 5 ?g/250 ?L of cell extract from 2.5 x 106 cells RIP - 15 ?g/500 ?L of cell extract from 4.5 x 106 cells

The eukaryote polypeptide chain initiation factor eIF4G forms complex with the mRNA cap-binding protein eIF4E and the ATP dependent RNA helicase eIF4A, constituting the initiation factor eIF4F complex. The eIF4G plays an important role in the mechanism of translation by tethering between other components of the ribosomal initiation factors. Two forms of eIF4G have been reported as two different gene products, eIF4G1 and 2. The eIF4G2 was identified as a functional homologue of eIF4G1. The homology of eIF4G2 to eIF4G1 is particularly high in central and carboxyl portions which corresponds to the eIF4A and eIF3 binding region. The proteolysis of both eIF4G1 and eIF4G2 is associated with a decrease in cell viability and the inhibition of protein synthesis.

1 mg/mL

Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.

200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 1982 Mouse: 13690

Peptide bond synthesis EVNPPSDETDSSSAPSKE KLH (783-800 aa)

This antibody reacts with human EIF4G2 (~102 kDa) on Western blotting, Immunoprecipitation and RNP Immunoprecipitation.

1 year

This antibody was purified from rabbit serum by affinity column chromatography. The rabbit was immunized with KLH conjugated synthetic peptide, EVNPPSDETDSSSAPSKE corresponding to 783-800 aa.

EIF4G2