Anti-ELAVL1 (HuR) (Human) pAb

WB - 1:1,000 for chemiluminescence detection system IP - 5 ?g/250 ?L of cell extract from 2.5 x 106 cells RIP - 15 ?g/500 ?L of cell extract from 1.5 x 107 cells

ELAVL1/HuR, like other ELAVL proteins, has a short N-terminal region followed by 2 RNA-binding motifs, a basic linker domain, and a third RNA-binding motif. The basic hinge region bears a novel nuclear shuttling sequence which is implicated in the nucleocytoplasmic HuR shuttling. Unlike its neuron-specific ELAV relatives (HuB, HuC and HuD), HuR itself is ubiquitously expressed. Structure-function analyses of HuR have shown that it binds to AU-rich mRNA sequences in the nucleus and may be involved in their nuclear export.

1 mg/mL

Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.

200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 1994 Mouse: 15568

Peptide bond synthesis MSNGYEDHMAEDCRGDIGR KLH (1-19 aa)

This antibody reacts with human ELAVL1 (~36 kDa) on Western blotting, Immunoprecipitation and RNP Immunoprecipitation.

1 year

This antibody was purified from rabbit serum by affinity column chromatography. The rabbit was immunized with KLH conjugated synthetic peptide, MSNGYEDHMAEDCRGDIGR corresponding to 1-19 aa.

ELAVL1/HuR