Anti-ELAVL2 (HuB) (Human) pAb

WB - 1:1,000 for chemiluminescence detection system IP - 5 ?g/250 ?L of cell extract from 2.5 x 106 cells RIP - 15 ?g/500 ?L of cell extract from 1.5 x 107 cells

ELAVL2/HuB is a neuron-specific RNA-binding protein, which contains three RNA recognition motifs (RRMs) and was found to bind in vitro to the 3? UTR of Id mRNA encoding a transcriptional repressor, which is abundantly expressed in undifferentiated neural precursors. Overexpression of ELAVL2/HuB can result in increased stability and translation of the ERG mRNA, which bears a U-rich region in its 3? UTR.

1 mg/mL

Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.

200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 1993 Mouse: 15569

Peptide bond synthesis CNNTANGPTTINNNCSSPVDSGNTEDSK KLH (11-38 aa)

This antibody reacts with human ELAVL2 (~39.5 kDa) on Western blotting, Immunoprecipitation and RNP Immunoprecipitation. Its ability to immunoprecipitate mRNP complex was confirmed by using transfectant.

1 year

This antibody was purified from rabbit serum by affinity column chromatography. The rabbit was immunized with KLH conjugated synthetic peptide, CNNTANGPTTINNNCSSPVDSGNTEDSK corresponding to 11-38 aa.

ELAVL2/HuB