Anti-EIF4E mAb

WB - 1 ug/mL (chemiluminescence detection system) IP - 1.5 ug/50 uL of cell extract from 4 x 105 cells/sample RIP - 15 ug/500 uL of cell extract from 4 x 106 cells/sample

The eukaryotic initiation factor 4E (eIF4E) is a key regulatory component of the mRNA cap-binding complex (eIF4F complex) that anchors eIF4F complex to the 5' end of capped mRNAs. eIF4F complex consists of eIF3, eIF4A, eIF4B, eIF4E, eIF4H and eIF4G. eIF3, the poly(A)-binding protein, and the eIF4 proteins recruit mRNA to the 43S initiation complex to form the 48S initiation complex. The eIF4 proteins consist of eIF4A; RNA helicase (46 kDa), eIF4B; RNA binding and RNA annealing protein (70 kDa), eIF4E (25 kDa); cap binding protein, eIF4H; work with eIF4B to stimulate eIF4A helicase activity (25 kDa), and eIF4G; co-localize all other proteins necessary for mRNA recruitment on the 40S subunit. As a principal initiation factor, eIF4E has the potential to influence expression of every protein in the cell. mRNA subset associated with eIF4E in p19 cell differed from mRNA subset associated with HuB or poly(A) binding protein, suggested that they reflect distinct functional subset of mRNAs whose expression is regulated differently at the level of translation.

1 mg/mL

Anti-EIF4E mAb (clone: C107-3-5) can be used for RIP, WB and IP.

PBS containing 50% Glycerol (pH 7.2). No preservative is contained.

Human: 1977 Mouse: 13684 Rat: 117045

1 year

Purified IgG from hybridoma supernatant

EIF4E