Anti-IGF2BP2 (IMP2) (Human) pAb

WB - 1:1,000 for chemiluminescence detection system IP - 5 ?g/250 ?L of cell extract from 2.5 x 106 cells RIP - 15 ?g/500 ?L of cell extract from 1.2 x 107 cells

IGF2BP2 is an RNA binding protein that posttranscriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated to in transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. IGF2BP family express tissue selectively. IGF2BP2 being expressed in fetal lung, kidney, thymus, placenta and fetal liver. Genetic valuation in IGF2BP2 was implicated to interact with Fetal Malnutrition on glucose metabolism.

1 mg/mL

Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.

200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 10644 Mouse: 319765

Peptide bond synthesis KQQEQKYPQGVASQRSK KLH (583-599 aa)

This antibody reacts with human IGF2BP2 (~66 kDa) on Western blotting, Immunoprecipitation and RNP Immunoprecipitation.

1 year

This antibody was purified from rabbit serum by affinity column chromatography. The rabbit was immunized with KLH conjugated synthetic peptide, KQQEQKYPQGVASQRSK corresponding to 583-599 aa.

IGF2BP2/IMP2