Anti-PABPC1 mAb
| Code | Size | Price |
|---|
| MBL-RN009M | 200 ul | £458.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 1E11
Regulatory Status: RUO
Target Species:
- Hamster
- Human
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 2 ug/500 uL of cell extract from 2.5 x 106 cells/sample ICC - 2-10 ug/mL RIP - 15 ug/500 uL of cell extract from 1.5 x 107 cells/sample
Background:
PABPC1, an RNA binding protein, binds to 3'-poly(A) tail of eukaryotic mRNA and plays multiple roles in post-transcriptional regulation of various RNAs, such as mRNA. PABPC1 functions as a stabilizer of mRNA containing poly(A) tail by binding to the element, while that also contributes to efficient translation of mRNA by forming a closed-loop structure through eIF4F complex, composed of eIF4E, eIF4A and eIF4G.
It has been also reported that PABPC1 is involved in mRNA processing, nuclear export, RNA decay and miRNA-mediated gene silencing.
Concentration:
1 mg/mL
Description:
PABPC1 is a poly(A)-binding protein which regulates post-transcriptional regulation of gene expression via binding to poly(A) tail of an RNA. RN009M (Clone 10E10) is useful for RIP, WB, IP and IC.
Formulation:
PBS containing 50% Glycerol (pH 7.2). No preservative is contained.
Gene IDs:
Human: 26986
Shelf Life:
1 year
Source:
Purified IgG from hybridoma supernatant
Target:
PABPC1
References
1) Thoma, C., et al., RNA 14, 1579-1589 (2008) [WB]
2) Battle, D. J., et al., J Biol Chem. 282, 27953-27959 (2007) [IC]
3) Goodier, J. L., et al., Mol Cell Biol. 27, 6469-83 (2007) [IC]
4) Sagliocco, F., et al., Biochem J. 400, 337-347 (2006) [WB]
5) Massenet, S., et al., Mol Cell Biol. 22, 6533-6541 (2002) [WB]
6) G?rlach, M., et al., Exp Cell Res. 211, 400-407 (1994)