Anti-STAU1 (Human) pAb

WB - 1:1,000 IP - 5 ug/250 uL of cell extract from 2.5 x 10⁶ cells RIP - 15 ug/500 uL of cell extract from 1.5 x 10⁷ cells

Mammalian Staufen1 (Stau1) is a ubiquitous double-stranded RNA binding protein associated with polysomes. It has two protein isoforms, molecular weights of 55 and 63 kDa. The 55 kDa isoform associates with 40S and 60S ribosomal subunit and colocalizes with the rough endoplasmic reticulum. Stau1 is thought to function in transporting mRNA, controlling and eliciting mRNA decay. Previous study showed that the Stau1 recruits upf1 to 3? UTR region of Arf1 mRNA and induces the Stau1-mediated mRNA decay (SMD). Recent study suggests that Stau1 regulates diverse class of mammalian transcripts. In order to identify mRNA targets of Staufen proteins, RIP-Assay technology was employed and identified distinct but overlapping subset of cellular mRNAs associated with Stau1 and Stau2-containing ribonucleoprotein complexes.

1 mg/mL

Posttranscriptional regu lation of gene expression is a ribonucleoprotein - driven process, which involves RNA binding proteins (RBPs) and non - coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation - Chip (RI P - Chip) , RIP - Seq and RIP - RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co - immunoprecipita t e the RNA binding protein a nd the associated su b set of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP - Certified Antibody is validated for use in RNP Immunoprecipitat i on (RIP) in conjunction with the RIP - Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT - PCR or microarray.

200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 6780 Mouse: 20853

Peptide bond synthesis MKLGKKPMYKPVDPYSRMQ KLH (82-100 aa)

This antibody reacts with human STAU1 (~63 kDa) on Western blotting, Immunoprecipitation and RNP Immunoprecipitation.

1 year

T his antibody was purified from rabbit serum by affinity column chromatography . The rabbit was immunized with KLH conjugated synthetic peptide, MKLGKKPMYKPVDPYSRMQ corresponding to 82 - 100 aa .

STAU1