Anti-Nono (P54NRB) mAb
| Code | Size | Price |
|---|
| MBL-RN013MW | 100 uL | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
- Hamster
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Alternative Names:
non-POU domain-containing octamer-binding protein nonA, NRB54, AA407051, AV149256
Applications:
WB - 1 µg/mL IP - 2-5 µg/sample ICC - 10 μg/mL IHC - 5 μg/mL
Background:
Nono, also known as p54nrb, localizes to nuclear paraspeckles composed of distinct membrane-less subnuclear organelles termed as nuclear bodies. Paraspeckles consist of some RNAs and proteins, and representative RNA component of paraspeckle is Neat1 long non-coding RNA, which can bind to Nono directly. Nono plays multiple roles in gene expression regulation systems such as transcription and splicing by binding to DNA and RNA via its coiled coil domain and RNA recognition motif, respectively. It is also reported that Nono relates to nuclear retention of “A-to-I edited” RNA.
Conjugate:
Unconjugated
Formulation:
1 mg/mL in PBS/50% glycerol, pH 7.2
Gene IDs:
Human: 53610 Mouse: 317259
Immunogen Translated:
KLH-conjugated synthetic peptide, CPPAFNRPAPGAE (corresponding to amino acid residues 453-464 of mouse Nono)
Target:
Nono (P54NRB)
References
- Imamura K et al. Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli. Mol Cell 53, 393-406 (2014) (PMID:24507715) PMID:
- Hirose T et al. NEAT1 long noncoding RNA regulates transcription via protein sequestration within subnuclear bodies. Mol Biol Cell 25,169-83 (2014) (PMID:24173718) PMID:
- Elzbieta K et al. Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Mol Cell Biol. 32, 4585-94 (2012) (PMID:22966205) PMID:
- Nakagawa S, Hirose T. Paraspeckle nuclear bodies--useful uselessness? Cell Mol Life Sci. 69, 3027–36 (2012) (PMID:22476590) PMID:
- Nakagawa S et al. Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice. J Cell Biol. 193, 31-9 (2011) (PMID:21444682) PMID:
- Kuwahara S et al. PSPC1, NONO, and SFPQ are expressed in mouse Sertoli cells and may function as coregulators of androgen receptor-mediated transcription. Biol Reprod. 75, 352-9 (2006) (PMID:16641145) PMID:
- Fox AH et al. Paraspeckles: a novel nuclear domain. Curr Biol. 12, 13-25 (2002) (PMID:11790299) PMID: