Anti-FMR1 (Human) pAb

WB - 1 ug/mL for chemiluminescence detection system IP - 5 ug/500 uL of cell extract from 5 x 106 cells RIP - 15 ug/500 uL of cell extract from 7 x 106 cells

The fragile X mental retardation 1 protein (FMR1) is a cytoplasmic RNA binding protein abundantly expressed in the brain neurons. The absence of FMR1 protein leads to fragile X syndrome associated with the expansion of trinucleotide 5?-d(CGG)-3? repeats within the FMR1 gene. Three RNA binding domains including 1 RGG box and 2 KH domains were found in FMR1. Besides the presence of RNA binding motifs, FMR1 was also found to carry nuclear localization and nuclear export signals and it is believed to shuttle in and out the nucleus. FMR1 associates with polyribosomes through RNA and it is suggested to act as both a negative regulator and an activator of mRNA translation. Furthermore, recent studies showed that FMR1 can associate with miRNA and the components of the miRNA pathway in vivo.

1 mg/mL

Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.

200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 2332 Mouse: 14265

C-terminal region of synthetic peptide binding KLH, human FMR1

This antibody reacts with human FMR1 on Western blotting, Immunoprecipitation and RNP Immunoprecipitation.

1 year

This antibody was purified from rabbit serum by affinity column chromatography. The rabbit was immunized with KLH conjugated synthetic peptide, corresponding to C-terminus of human FMR1.

FMR1