Anti-HNRNPK (Human) pAb

WB - 1 ug/mL for chemiluminescence detection system IP - 5 ug/500 uL of cell extract from 5 x 106 cells RIP - 15 ug/500 uL of cell extract from 1.5 x 107 cells

The heterogeneous nuclear ribonucleoprotein K (hnRNP K), is an RNA-binding protein containing 3 KH RNA-binding domains. It binds to both RNA and DNA and is a component of the hnRNP complex. hnRNP K is associated with pre-mRNA and appears to influence the processing of pre-mRNAs and metabolism of mRNA. hnRNP K is predominantly localized at the nucleus and shuttles between the nucleus and the cytoplasm. Therefore, hnRNP K is thought to be involved in the transport of mRNA. Phosphorylation of hnRNP K leads to its cytoplasmic accumulation and is important for cell migration and metastasis. In the cytoplasm, hnRNP K functions as a translational regulator of specific mRNAs such as c-myc mRNA, 16L2 mRNA, and r15-LOX mRNA. Further, it specifically associates with c-src kinase, leading to c-src activation and hnRNP K phosphorylation, which affect the binding of hnRNP K to RNA.

1 mg/mL

Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.

200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 3190 Mouse: 15387

C-terminal region of synthetic peptide binding KLH, human HNRNPK

This antibody reacts with human HNRNPK on Western blotting, Immunoprecipitation and RNP Immunoprecipitation.

1 year

This antibody was purified from rabbit serum by affinity column chromatography. The rabbit was immunized with KLH conjugated synthetic peptide, corresponding to C-terminus of human HNRNPK.

HNRNPK