Anti-CIRBP (Human) pAb

WB - 1 ug/mL for chemiluminescence detection system IP - 5 ug/500 uL of cell extract from 5 x 106 cells RIP - 15 ug/500 uL of cell extract from 2 x 107 cells

The cold-inducible RNA-binding protein (CIRBP) contains an N-terminal RNA binding domain and a C-terminal Gly-rich domain. It exhibits specific RNA-binding activity owing to its ability to bind to poly (U) even at high NaCl concentrations. CIRBP transcripts are expressed in cells such as K562, HepG2, HeLa, and T24. In all of them, the transcript and translation levels of CIRBP were increased when the culture temperature was lowered from 37 to 32 oC. CIRBP affects gene expression by facilitating translation under mild cold stress, and it may play an essential role in cold-induced suppression of cell proliferation.

1 mg/mL

Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.

200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 1153 Mouse: 12696 Rat: 81825

N-terminal region of synthetic peptide binding KLH, human CIRBP

This antibody reacts with human CIRBP on Western blotting, Immunoprecipitation and RNP Immunoprecipitation.

1 year

This antibody was purified from rabbit serum by affinity column chromatography. The rabbit was immunized with KLH conjugated synthetic peptide, corresponding to N-terminus of human CIRBP.

CIRBP