Anti-TNRC6A (GW182) (Human) pAb

WB - 1 ug/mL for chemiluminescence detection system IP - 5 ug/500 uL of cell extract from 1 x 107 cells RIP - 15 ug/500 uL of cell extract from 1 x 107 cells

The trinucleotide repeat containing 6A (TNRC6A), also known as GW182, was so named because of its molecular weight and the presence of multiple glycine (G)?tryptophan (W) amino acid pairs in the N-terminal region that is required for interaction with Argonaute proteins. GW182 is a critical component of cytoplasmic processing bodies (P-bodies), which have been shown to function in mRNA degradation, storage, and miRNA-mediated gene silencing. The interaction of GW182 with Argonautes is required for P-body localization and may be critical for miRNA-mediated translational repression, because it induces the formation of the complexes containing miRNA?target mRNA. Further, the phosphorylated form of human TNRC6A has been shown to play a role in miRNA mediated gene silencing.

1 mg/mL

Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of RNAs. The RNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit for microRNA distributed from MBLI. Its ability to immunoprecipitate RNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray.

200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 27327

Internal region synthetic peptide binding KLH, human TNRC6A

This antibody reacts with human TNRC6A on Western blotting, Immunoprecipitation and RNP Immunoprecipitation.

1 year

This antibody was purified from rabbit serum by affinity column chromatography. The rabbit was immunized with KLH conjugated synthetic peptide, corresponding to internal region of human TNRC6A.

TNRC6A