MS Mouse LEP(Leptin) ELISA Kit
| Code | Size | Price |
|---|
| E-MSEL-M0033-24T | 24T | £186.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| E-MSEL-M0033-48T | 48T | £281.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
| E-MSEL-M0033-96T | 96T | £350.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month store the items separately according to the following conditions once the kit is received.
Further Information
Abbreviation:
LEP
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Mouse LEP in samples.No significant cross-reactivity or interference between Mouse LEP and analogues was observed
Detection Range:
0.63-40 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.38 ng/mL
Target Species:
Mouse
Target Synonym:
LEP
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P41160
Usage:
This ELISA kit applies to the in vitro quantitative determination of Mouse LEP concentrations in serum, plasma and other biological fluids.
References
- https://academic.oup.com/biomedgerontology/article-abstract/76/3/393/5813438
- https://www.sciencedirect.com/science/article/pii/S0891584918314394
- https://www.sciencedirect.com/science/article/pii/S0269749120363867
- https://www.mdpi.com/1042904
- https://www.sciencedirect.com/science/article/pii/S0045653519329017
- https://www.mdpi.com/1422-0067/21/14/5121
- https://www.tandfonline.com/doi/abs/10.1080/13813455.2021.1894178
- https://www.mdpi.com/2218-273X/10/10/1454
- https://pubs.rsc.org/en/content/articlelanding/2020/fo/d0fo01405b
- https://www.sciencedirect.com/science/article/pii/S1756464619300854
- https://www.sciencedirect.com/science/article/pii/S001429992030296X
- https://www.sciencedirect.com/science/article/pii/S0024320520301909