Rat SELP(P-Selectin) ELISA Kit

Elabscience
Product Code: E-EL-R3045
Product Group: ELISA Kits
Supplier: Elabscience
CodeSizePrice
E-EL-R3045-24T24T£207.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-EL-R3045-48T48T£321.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
E-EL-R3045-96T96T£403.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month store the items separately according to the following conditions once the kit is received.

Images

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Standard curve

Standard curve

Documents

Further Information

Abbreviation:
SELP
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Rat SELP in samples.No significant cross-reactivity or interference between Rat SELP and analogues was observed
Detection Range:
78.13-5000 pg/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
46.88 pg/mL
Target Species:
Rat
Target Synonym:
IL1R2;CD121b;CDw121b;IL-1R-2;IL-1RT-2;IL-1RT2;IL1R2c;IL1RB;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Rat SELP. You can calculate the concentration of Rat SELP in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P98106
Usage:
This ELISA kit applies to the in vitro quantitative determination of Rat SELP concentrations in serum, plasma and other biological fluids.

References

  • https://www.sciencedirect.com/science/article/pii/S001429992100707X
  • https://www.sciencedirect.com/science/article/pii/S0753332218344548
  • https://pubs.acs.org/doi/abs/10.1021/acsomega.0c05227
  • https://www.tandfonline.com/doi/abs/10.1080/08916934.2019.1575820
  • https://link.springer.com/article/10.1007/s11033-020-05746-3