Recombinant MCM2 Monoclonal Antibody

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Elabscience
Product Code: E-AB-81581
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-81581-50uL50uL£184.00
Quantity:
E-AB-81581-100uL100uL£251.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: R08-9H4
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot detection of MCM2 in K562,C6,3T3,Hela cell lysates using MCM2 Rabbit mAb(1:1000 diluted).Predicted band size:102kDa.Observed band size:125kDa.
2 / 2
Immunofluorescence of MCM2(green) in Hela cells using MCM2 Rabbit mAb at dilution 1:50, and DAPI(blue)
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Western blot detection of MCM2 in K562,C6,3T3,Hela cell lysates using MCM2 Rabbit mAb(1:1000 diluted).Predicted band size:102kDa.Observed band size:125kDa.
Immunofluorescence of MCM2(green) in Hela cells using MCM2 Rabbit mAb at dilution 1:50, and DAPI(blue)

Documents

Further Information

Abbreviation:
MCM2
Background:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with mCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Buffer:
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Calculated MW:
102kDa
Concentration:
300 ug/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:1000 IF 1:50-1:200
Immunogen:
A synthetic peptide of human MCM2
ObservedMW:
125kDa
Purification method:
Affinity Purified
Target Synonym:
BM28;CCNL 1;CCNL1;CDC like 1;cdc19;CDCL 1;CDCL1;Cell devision cycle like 1;Cyclin like 1;D3S3194;DNA replication licensing factor MCM2;KIAA0030;MCM 2;MCM2;MCM2 minichromosome maintenance deficient 2 mitotin;MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae);MCM2 minichromosome maintenance deficient 2;mitotin;MCM2;MGC10606;Minichromosome maintenance complex component 2;Minichromosome maintenance deficient 2 (mitotin);Minichromosome maintenance deficient 2 mitotin;Minichromosome maintenance protein 2;Minichromosome maintenance protein 2 homolog;Mitotin;Nuclear protein BM28;OTTHUMP00000216047;OTTHUMP00000216050
UNIProt ID:
P49736