Recombinant TRIM25 Monoclonal Antibody

Elabscience
Product Code: E-AB-81492
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-81492-50uL50uL£184.00
Quantity:
E-AB-81492-100uL100uL£251.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: R06-1H2
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot detection of TRIM25/EFP in K562,Rat Brain,C6,Hela cell lysates using TRIM25/EFP Rabbit mAb(1:1000 diluted).Predicted band size:71kDa.Observed band size:71kDa.
2 / 2
Immunofluorescence of TRIM25/EFP (green) in MCF-7 using TRIM25/EFP antibody at dilution 1:20, and DAPI(blue)

Western blot detection of TRIM25/EFP in K562,Rat Brain,C6,Hela cell lysates using TRIM25/EFP Rabbit mAb(1:1000 diluted).Predicted band size:71kDa.Observed band size:71kDa.
Immunofluorescence of TRIM25/EFP (green) in MCF-7 using TRIM25/EFP antibody at dilution 1:20, and DAPI(blue)

Documents

Further Information

Abbreviation:
TRIM25
Background:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Buffer:
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Calculated MW:
71kDa
Concentration:
300 ug/mL
Conjugation:
Unconjugated
Dilution:
WB 1:1000-1:5000 IF 1:20-1:100
Immunogen:
A synthetic peptide of human TRIM25
ObservedMW:
71kDa
Purification method:
Affinity Purified
Target Synonym:
E3 ubiquitin/ISG15 ligase TRIM25;EFP;Estrogen responsive finger protein;Estrogen-responsive finger protein;RING finger protein 147;RNF 147;RNF147;TRI25;TRI25;TRIM 25;Trim25;Tripartite motif containing 25;Tripartite motif containing protein 25;Tripartite motif protein TRIM25;Tripartite motif-containing protein 25;Tripartite motifcontaining25;Ubiquitin/ISG15-conjugating enzyme TRIM25;Z147;Zinc finger protein 147 (estrogen responsive finger protein);Zinc finger protein 147;Zinc finger protein-147;ZNF 147;ZNF147
UNIProt ID:
Q14258