GAPDH Monoclonal Antibody

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Elabscience
Product Code: E-AB-48016
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-48016-20uL20uL£110.00
Quantity:
E-AB-48016-50uL50uL£143.00
Quantity:
E-AB-48016-100uL100uL£177.00
Quantity:
E-AB-48016-200uL200uL£210.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3B3
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Caprine (goat)
  • Cavia (Guinea pig)
  • Human
  • Porcine (pig)
  • Rabbit
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000. Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate. Lysates/proteins at 20g per lane.
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Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000. Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate. Lysates/proteins at 20g per lane.

Documents

Further Information

Abbreviation:
GAPDH
Background:
GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Calculated MW:
36 kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB: 1:5000-10000
Immunogen:
Recombinant human GAPDH protein expressed by E.coli
ObservedMW:
36 kDa
Purification method:
Protein A/G Purified
Target Synonym:
aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component
UNIProt ID:
Q5XJ10

References

  • https://pubmed.ncbi.nlm.nih.gov/35194032/
  • https://www.sciencedirect.com/science/article/pii/S2452199X21004874
  • https://pubmed.ncbi.nlm.nih.gov/36512628/
  • https://www.sciencedirect.com/science/article/pii/S2590006422001405
  • https://www.sciencedirect.com/science/article/pii/S0142961219303473
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295061/
  • https://www.sciencedirect.com/science/article/pii/S0753332223000823
  • https://www.sciencedirect.com/science/article/pii/S0753332222012331
  • https://www.nature.com/articles/s41401-022-01045-8
  • https://www.mdpi.com/2161010
  • https://www.frontiersin.org/articles/10.3389/fonc.2021.727605/full
  • https://www.sciencedirect.com/science/article/pii/S0891584920313022