Flag-Tag Polyclonal Antibody

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Elabscience
Product Code: E-AB-40525
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-40525-20uL20uL£110.00
Quantity:
E-AB-40525-50uL50uL£143.00
Quantity:
E-AB-40525-100uL100uL£177.00
Quantity:
E-AB-40525-200uL200uL£210.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunofluorescent analysis of Hela cells transfected with the plasmid overexpressing Flag fusion Protein, using anti-Flag -Tag polyclonal antibody at 1:200 dilution.
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Immunofluorescent analysis of Hela cells transfected with the plasmid overexpressing Flag fusion Protein, using anti-Flag -Tag polyclonal antibody at 1:200 dilution.

Documents

Further Information

Abbreviation:
Flag-Tag
Background:
FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This
Buffer:
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Calculated MW:
28kDa
Concentration:
4mg/mL
Conjugation:
Unconjugated
Dilution:
WB: 1:2000-5000; IP: 3ug/sample; IF: 1:6000-12000
Immunogen:
Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.
ObservedMW:
28kDa
Purification method:
Antigen Affinity Purification
Target Synonym:
DDDDK epitope tag;DDDDK epitope tag

References

  • https://pubmed.ncbi.nlm.nih.gov/31455039/