His-Tag Polyclonal Antibody

Elabscience
Product Code: E-AB-40506
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-40506-20uL20uL£110.00
Quantity:
E-AB-40506-50uL50uL£143.00
Quantity:
E-AB-40506-100uL100uL£177.00
Quantity:
E-AB-40506-200uL200uL£210.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Species Independent
Application: Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot with anti-6xHis antibody at dilution of 1:2000. 
 lane 1: HEK 293 whole cell lysate, lane 2: 6xHis tag transfected HEK 293 cell lysates

Western blot with anti-6xHis antibody at dilution of 1:2000. 
 lane 1: HEK 293 whole cell lysate, lane 2: 6xHis tag transfected HEK 293 cell lysates

Documents

Further Information

Abbreviation:
His-Tag
Background:
The His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. It is the most widely used protein affinity tag. Due to its small size of only six amino acids it rarely in
Buffer:
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Calculated MW:
28kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB: 1:1000-2000
Immunogen:
Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin.
ObservedMW:
28kDa
Purification method:
Antigen Affinity Purification
Target Synonym:
6His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag;His tag; Polyhistidine Tag