Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K097-S-50Assays | 50Assays | £210.00 |
Quantity:
| E-BC-K097-S-100Assays | 100Assays | £264.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
See the manual
Further Information
Assay Time:
180 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.12-30 umol/L T-GSH
Detection Type:
Colorimetric method
Inter cv:
4.7
Intra cv:
0.9
Result Type:
Quantitative
Sensitivity:
0.12 umol/L T-GSH
Target Synonym:
T-GSH;GSSG
Test Principle:
GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.
Tested Sample Types:
Serum,plasma,animal tissue,red blood cells,cultured cells
References
- https://www.sciencedirect.com/science/article/pii/S0308814621004301
- https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03577k
- https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.15397
- https://www.tandfonline.com/doi/abs/10.1080/13880209.2022.2070221
- https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-022-00275-w
- https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.11611
- https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.2024319