Vitamin C (VC) Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K034-S-50Assays | 50Assays | £177.00 |
Quantity:
| E-BC-K034-S-100Assays | 100Assays | £210.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
80 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.35-20 ug/mL
Detection Type:
Colorimetric method
Inter cv:
3.3
Intra cv:
2.3
Result Type:
Quantitative
Sensitivity:
0.35 ug/mL
Target Synonym:
VC;Ascorbic acid
Test Principle:
The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.
Tested Sample Types:
Serum,plasma,tissue
References
- https://www.mdpi.com/article/10.3390/nu15040832
- https://www.sciencedirect.com/science/article/pii/S0891584920310911
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/
- https://www.nature.com/articles/s41598-019-52646-z
- https://www.sciencedirect.com/science/article/pii/S0024320520303325
- https://www.tandfonline.com/doi/abs/10.2147/IJGM.S402206
- https://onlinelibrary.wiley.com/doi/abs/10.1002/jez.2603