Total Oxidant Status (TOS) Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K802-M-48T | 48T | £143.00 |
Quantity:
| E-BC-K802-M-96T | 96T | £177.00 |
Quantity:
| E-BC-K802-M-500Assays | 500Assays | £378.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Range:
2.5-100 umol H2O2 Equiv./L
Detection Type:
Colorimetric method
Inter cv:
3.5
Intra cv:
2.3
Result Type:
Quantitative
Sensitivity:
2.5 umol H2O2 Equiv./L
Target Synonym:
TOS
Test Principle:
Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample.
Tested Sample Types:
cell, tissue, serum and other liquid samples
References
- https://www.hindawi.com/journals/omcl/2022/4591134/
- https://www.mdpi.com/2079-7737/10/9/820
- https://onlinelibrary.wiley.com/doi/abs/10.1002/jbt.23083
- https://link.springer.com/article/10.1007/s12011-022-03275-7