Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method)
| Code | Size | Price |
|---|
| E-BC-K329-S-50Assays | 50Assays | £157.00 |
Quantity:
| E-BC-K329-S-100Assays | 100Assays | £177.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
60 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.12-15 mmol/L
Detection Type:
Colorimetric method
Inter cv:
9.9
Intra cv:
4.9
Result Type:
Quantitative
Sensitivity:
0.12 mmol/L
Target Synonym:
BUN
Test Principle:
In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:
Tested Sample Types:
Serum,plasma,saliva,urine
References
- https://www.sciencedirect.com/science/article/pii/S1742706121007108
- https://www.hindawi.com/journals/omcl/2022/1763922/
- https://www.mdpi.com/2124124
- https://www.mdpi.com/article/10.3390/ijms232315400
- https://europepmc.org/article/med/35392552?javascript_support=no
- https://www.sciencedirect.com/science/article/pii/S0753332220308775
- https://www.tandfonline.com/doi/abs/10.1080/09603123.2022.2105824
- https://www.frontiersin.org/articles/10.3389/fphys.2023.1083725/full?utm_source=dlvr.it&utm_medium=twitter
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525417/
- https://academic.oup.com/jpp/advance-article-abstract/doi/10.1093/jpp/rgab078/6299670
- https://www.mdpi.com/1433254