Ferrous Ion Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K304-S-50Assays | 50Assays | £177.00 |
Quantity:
| E-BC-K304-S-100Assays | 100 Assays | £224.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
45 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.08-20 mg/L
Detection Type:
Colorimetric method
Inter cv:
3.2
Intra cv:
3
Result Type:
Quantitative
Sensitivity:
0.08 mg/L
Test Principle:
Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.
Tested Sample Types:
Serum, plasma, animal tissue
References
- https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01281-4
- https://www.hindawi.com/journals/omcl/2022/9735555/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124810/
- https://www.sciencedirect.com/science/article/pii/S0014488622001960
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8936435/
- https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/s12986-022-00715-y
- https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.2010369
- https://www.spandidos-publications.com/10.3892/ol.2022.13281