L-Lactic Acid/Lactate (LA) Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K044-S-50Assays | 50Assays | £224.00 |
Quantity:
| E-BC-K044-S-100Assays | 100Assays | £277.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
60 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.05-6.0 mmol/L
Detection Type:
Colorimetric method
Inter cv:
1.9
Intra cv:
1.1
Result Type:
Quantitative
Sensitivity:
0.05 mmol/L
Test Principle:
Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.
Tested Sample Types:
Serum,plasma,cell culture supernatant,tissue,cells
References
- https://link.springer.com/article/10.1186/s12964-022-00882-8
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207367/
- https://www.sciencedirect.com/science/article/pii/S258900422101138X
- https://www.sciencedirect.com/science/article/pii/S0161813X21001224
- https://pubs.acs.org/doi/abs/10.1021/acsomega.2c01494
- https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.1980644
- https://link.springer.com/article/10.1007/s12010-023-04373-5
- https://link.springer.com/article/10.1007/s10863-022-09938-3
- https://www.spandidos-publications.com/10.3892/mmr.2021.12103
- https://biomedical-engineering-online.biomedcentral.com/articles/10.1186/s12938-021-00903-6