SARS-CoV-2 Spike Protein S1 RBD ELISA Kit
| Code | Size | Price |
|---|
| E-EL-E605-96T | 96T | £371.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing! | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
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Overview
Host Type: Virus
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month store the items separately according to the storage table in the manual once the kit is received.
Further Information
Abbreviation:
S1RBD
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Human S1 RBD in samples.No significant cross-reactivity or interference between Human S1 RBD and analogues was observed
Detection Range:
0.39-25 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.09 ng/mL
Target Species:
Human
Target Synonym:
Spike protein S1;S1 subunit;Receptor binding domain;S1RBD;RBD domain;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SARS-CoV-2 S1RBD. Samples (or Standards) and biotinylated detection antibody specific for SARS-CoV-2 S1RBD are added to the micro ELISA plate wells. SARS-CoV-2 S1RBD would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 S1RBD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ? 2 nm. The OD value is proportional to the concentration of SARS-CoV-2 S1RBD. You can calculate the concentration of SARS-CoV-2 S1RBD in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P0DTC2
Usage:
This ELISA kit applies to the in vitro Qualitative determination of S1 RBD in serum or plasma.
References
- https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202100316
- https://www.nature.com/articles/s41598-022-12252-y